EFFECTS OF ALLIUM SATIVUM LINN. (SOLO GARLIC) EXTRACT IN INCREASING SUPEROXIDE DISMUTASE (SOD) LEVELS IN RATTUS NORVEGICUS STRAIN WISTAR EXPOSURED BY E-CIGARETTE

Abstract

ABSTRACT

Background: The popularity of E-Cigarette has increased significantly over the last decade, and has potential to replace tobacco cigarettes. Aerosol which is produced by E-Cigarette, contains reactive carbonyl and free radicals, which is inducing oxidative stress. This oxidative stress can be seen through the Malondialdehyde (MDA) levels in the body, and reduced by antioxidant activity in Solo Garlic extract through Superoxide Dismutase (SOD) enzyme activity.

Materials and Methods: The aim of this study is analyzing the differences levels of SOD in four treatment groups. Twenty eight white rats were divided into four groups, three of them were exposured by E-Cigarette, and another one was a control group. Two treatment groups were given solo garlic extract dose 0,05 gram/day and 0,10 gram/day. The average of SOD levels in these four treatment groups was analyzed using One Way ANOVA test.

Result: This study showed that the lowest SOD levels was found in group which was exposured by E-Cigarette only, amount 88,431 U/ml. SOD levels can be increased to 93,814 U/ml by the lowest dose of solo garlic extract (0,05 gram/day), which is higher than the control group (92,669 U/ml).

Conclusion: It can be concluded, that using solo garlic extract less than 0,05 gram/day can restore SOD levels due to E-Cigarette exposure to a normal state.

Keywords: E-Cigarette, Superoxide Dismutase (SOD), and  Allium sativum Linn. (Solo Garlic).