EFFECTS OF BEAN JUICE (Phaseolus vulgaris Linn) FOR INCREASING SUPEROXIDE DISMUTASE ACTIVITY IN PSYCHOLOGICAL STRESS RATS
Background: Psychological stress is often suffered to people with body rhythm disorders, such as in sleep disorders upside down people. Psychological stress can trigger oxidative stress. Oxidative stress can be minimized by antioxidant activity. Beans as antioxidants are considered to reduce oxidative stress. The aim of this study was to examine the effectiveness of bean juice in increasing SOD activity in male white rats (strain wistar) exposed by psychological stress.
Materials and Methods: This study is an experimental research, with Randomized Post Test Only Group Design. There were five groups in this study, control group, stress control group, and 3 treatment groups. Treatment groups were exposure by psychological stress, which was given by inverted sleep guard pattern for 1 x 24 hours. These treatment groups were given bean juice dose 4,5 g/ml. Blood from each treatment group was taken after 1 hour, 6 hours, and 24 hours to measure SOD activity. MANOVA (Multivariate Analysis of Variance) Test was used to analyze the difference of SOD activity in five treatment groups.
Result: The results showed that SOD activity can be increased by providing bean juice in the first hour after psychological stress exposure. The highest SOD level was found after 24 hours giving bean juice. SOD activity can increase due to the content of flavonoids in beans that can fight free radicals and increase endogenous antioxidants activity through scavening mechanism.
Conclusion: A single dose of bean juice in this study, can increase SOD activity after one hour giving by bean juice and its antioxidant activity persisted until 24th hour. It showed that bean juice can work as an antioxidant by increasing SOD activity.Keywords: oxidative stress, psychological stress, bean juice, Phaseolus vulgaris Linn, malondialdehyde